Growth Hormone 1 Kit ELISA

N° du produit ABIN6720046

N° du produit (Fournisseur): koa0354

Kit ELISA Humain Growth Hormone 1, test Colorimetric pour la quantification de Humain Growth Hormone 1.

Aperçu rapide pour Growth Hormone 1 Kit ELISA (ABIN6720046)

Antigène: Growth Hormone 1 (GH1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit Growth Hormone 1 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0354

Fournisseur: Rockland

Fonction: Human Growth Hormone Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human GH. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human GH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human GH standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: F27-F217

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Growth Hormone 1 (GH1)

Autre désignation: Growth Hormone

Sujet:

Synonyms: gH, GH-N, GH1, GHN, Growth hormone, Growth hormone 1, HGH, Pituitary growth hormone, SOMA_HUMAN, Somatotropin

Background: Growth hormone(GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone(GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency(GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.

ID gène: 2688

NCBI Accession: NP_000506

UniProt: B1A4G6

Pathways: Signalisation NF-kappaB, Signalistation JAK/STAT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process